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Anti-Mouse IgG kappa, AlpSdAbs® VHH(PE)

Details and Advantages
Applications: Flow Cyt
Reactivity: Mouse IgG kappa chain
Conjugate: PE
Advantages:

Consistent and reproducible results;

· Extraordinarybinding,even under harsh conditions;

· High affinity and Short incubation (5-30min)

概述 >

Description:
Anti-Mouse IgG kappa, AlpSdAbs® VHH(PE) is designed for detecting mouse IgG kappa chain specifically. Anti-Mouse IgG kappa, AlpSdAbs® VHH(PE) is based on recombinant single domain antibody to mouse IgG kappa chain coupled to PE. Based on immunoelectrophoresis and/or ELISA, Anti-Mouse IgG kappa, AlpSdAbs® VHH(PE) reacts with the mouse IgG kappa chain selectively, no reactivity with the mouse IgG lambda chain or heavy chain of mouse IgG.

Immunogen: Fab region of Mouse IgG                  
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c    
Conjugate: PE(Ex: 488nm, Em: 575nm)  
Specificity: Mouse IgG kappa chain
Cross-Reactivity: No cross-reactivity with rabbit, human, cynomolgus, rat, goat IgG    
Purity: Recombinant Expression and Affinity purified
Concentration: 0.1mg/mL
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles) , Protect from light.

Background:
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH.

性能 >

Flow Cyt:  1:200-1:1000
ICC/IF:  1:200-1:1000


Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.