Anti-FMC63, AlpHcAbs® Rabbit antibody(PE)

Details and Advantages

                                                Applications:
                                                Flow Cyt
                                            
                                                Reactivity:
                                                FMC63
                                            
                                                Conjugate:
                                                PE
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

概述 >

Description:
Anti-FMC63, AlpHcAbs® Rabbit antibody(PE) is designed for detecting FMC63 scFv antibody specifically. Anti-FMC63, AlpHcAbs® Rabbit antibody(PE) is based on monoclonal, recombinant single domain antibody to FMC63 antibody fused to PE. Based on immunoelectrophoresis and/or ELISA, Anti-FMC63, AlpHcAbs® Rabbit antibody(PE) reacts with the reacts with the FMC63 scFv antibody specifically.

Immunogen: FMC63 antibody
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to Rabbit IgG Fc
Conjugate: PE(Ex: 488nm, Em: 575nm)
Specificity: FMC63 scFv antibody
Cross-Reactivity: Recognizes FMC63 antibody, No Cross-reactivity to mouse IgG
Purity: Recombinant Expression and Affinity purified
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at 2-8 °C, Protect from light.

Background:
The FMC63 scFv (single-chain variable fragment) is a key component in many chimeric antigen receptor (CAR) designs, particularly in CAR-T cell therapies. Derived from a murine monoclonal antibody, FMC63 specifically targets human CD19, a cell surface protein highly expressed on B-cells, including malignant B-cells in B-cell leukemias and lymphomas. This scFv fragment is engineered by fusing the variable regions of the heavy and light chains of the antibody, preserving the antigen-binding capability while significantly reducing its size. This smaller, single-chain format enhances its integration into CAR constructs, enabling the recognition and destruction of CD19-positive cells by CAR-T cells. FMC63-based CARs are widely used in immunotherapy, particularly in FDA-approved treatments for B-cell malignancies, such as Kymriah® and Yescarta®.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

性能 >

Flow Cytometry Detection

(1) Take 5×10^5 test cells, wash them twice with pre-cooled FACS Buffer, and centrifuge at 1500 rpm for 5 minutes each time, Finally, resuspend with 100 μL FACS Buffer;

(2) Add 1 μL of Anti-FMC63, AlpHcAbs® Rabbit antibody(PE) solution to the cell suspension and mix well. Incubate in the dark at 2-8°C for 30-60 minutes

(3)After incubation, wash twice with pre-cooled FACS Buffer, then resuspend in 200 μL FACS Buffer for machine detection.

*FACS Buffer:1×PBS(pH7.4), 2%BSA

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.