Tag related peptides are essential tools for studying antibody (Abs) or protein function and localization. They can efficiently purify tag fusion proteins from cell lysate or complex mixtures, and are often used in combination with affinity chromatography columns to obtain high-purity target proteins for subsequent immunoprecipitation (IP), Western Blot detection, protein function research, drug screening and development, and other directions.

Advantage:

Mild conditions preserve protein activity: Peptide elution is usually carried out in neutral buffer, avoiding the destruction of protein structure and activity by acidic or denaturing conditions, especially suitable for proteins with high activity requirements, such as enzymes, receptors, etc.

High specificity and low background: The binding between peptides and anti-tag antibodies has high specificity, which can effectively competitively elute target proteins, reduce interference from non-specific binding proteins, and improve the purity and signal-to-noise ratio of purification or immunoprecipitation.

Easy and fast operation: No complex chemical treatment or extreme conditions are required. The elution process is usually carried out at room temperature or low temperature, with short operation time, suitable for high-throughput experiments.

Avoiding antibody contamination: The eluted sample does not contain light and heavy chains of anti-Tag antibodies, which avoids interference from antibody bands in subsequent detection (such as Western Blot) and facilitates more accurate analysis of target proteins.