Anti-GGGGS Linker, AlpHcAbs® Rabbit antibody(PE)

Details and Advantages

                                                Applications:
                                                Flow Cyt
                                            
                                                Reactivity:
                                                GGGGS Linker
                                            
                                                Conjugate:
                                                PE
                                            
                                                Advantages:
                                                High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

概述 >

Description:
Anti-GGGGS Linker, AlpHcAbs® Rabbit antibody(PE) is designed for detecting GGGGS linker specifically. Anti-GGGGS Linker, AlpHcAbs® Rabbit antibody(PE) is recombinant antibody coupled to PE. Based on immunoelectrophoresis and/or ELISA, Anti-GGGGS Linker, AlpHcAbs® Rabbit antibody(PE) reacts with GGGGS linker and their fusion proteins.

Immunogen: Synthetic peptide(GGGGSGGGGSGGGGS)
Host: Rabbit
Isotype: Rabbit IgG
Conjugate: PE(Ex: 488nm, Em: 575nm)
Specificity: This product recognizes (GnS)m linker(n≥2, m≥2) in proteins, antibodies or cells, such as (G2S)2 linker, (G2S)4 linker, (G3S)3 linker, (G4S)2 linker and (G4S)3 linker, etc.
Cross-Reactivity: N/A
Purity: Recombinant Expression and Affinity purified
Concentration: 0.2mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at 4 °C(Avoid freeze / thaw cycles), protect from light

Background:
As a crucial element in the design of recombinant fusion proteins, linkers play an increasingly vital role in the construction of stable, bioactive fusion proteins. GGGGS linker (G4S linker) is a flexible linker made of 4 glycine repeats followed by a serine amino acid. Due to its flexibility and resistance to proteases, GGGGS and its repeats are commonly used when engineering a protein, particularly in the construction of single-chain Fv(ScFv) domains expressed on the surfaces of CAR-T cells.

性能 >

Flow Cytometry Detection

(1) Take 5×10^5 test cells, wash them twice with pre-cooled FACS Buffer, and centrifuge at 1500 rpm for 5 minutes each time, Finally, resuspend with 100 μL FACS Buffer;

(2) Add 1 μL of Anti-GGGGS Linker, AlpHcAbs® Rabbit antibody(PE) solution to the cell suspension and mix well. Incubate in the dark at 2-8°C for 30 minutes

(3)After incubation, wash twice with pre-cooled FACS Buffer, then resuspend in 200 μL FACS Buffer for machine detection.

*FACS Buffer:1×PBS(pH7.4), 2%BSA

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.