Human CD7-PE CAR TEST Kit - 临界点生物

Details and Advantages

                                                Applications:
                                                Flow Cyt
                                            
                                                Reactivity:
                                                Human CD7
                                            
                                                Conjugate:
                                                PE
                                            
                                                Advantages:
                                                High lot-to-lot consistency

概述 >

Description:
Human CD7-PE CAR TEST Kit is designed for detecting antibody expression of CAR related cell specifically. Human CD7-PE CAR TEST Kit is recombinant human CD7 antigen fused to PE. Based on FCM and/or ELISA, CD7-PE CAR TEST Kit reacts with Anti-human CD7 antibody and their chimerism.

Protein Construction：Human CD7(Ala26-Pro180) conjugated to PE
Expression System：CHO
Purity: Recombinant Expression and Affinity purified
Conjugate: PE(Ex: 488nm, Em: 575nm)
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at 4°C(Avoid freeze / thaw cycles), protect from light

Background:
T-Cell Antigen CD7 is a single-pass type I membrane protein that that belongs to the the immunoglobulin superfamily. Human CD7 is synthesized as a 240 amino acid precursor that contains a 25 amino acid signal sequence and a 215 amino acid mature chain with a Ig-like (immunoglobulin-like) domain. CD7 is normally expressed on all T-lymphocytes, NK-cells, pre-B lymphocytes and pleuripotent hematopoietic stem cells. CD7 plays an essential role in T-cell interactions, T-cell/B-cell interaction during early lymphoid development, T- and NK-cell activation and cytokine production. CD7 has been shown to interact with PIK3R1and SECTM1. However, the function of the CD7 protein in the immune system is still largely unknown.

性能 >

Flow Cytometry Detection

(1) Take 5×10^5 test cells, wash them twice with pre-cooled FACS Buffer, and centrifuge at 1500 rpm for 5 minutes each time, Finally, resuspend with 100 μL FACS Buffer;

(2) Add 2 μL of Human CD7-PE solution to the cell suspension and mix well. Incubate in the dark at 2-8°C for 30 minutes

(3)After incubation, wash twice with pre-cooled FACS Buffer, then resuspend in 200 μL FACS Buffer for machine detection.

*FACS Buffer:1×PBS(pH7.4), 2%BSA

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.