High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Description:
Anti-RFP, AlpHcAbs® Human IgG antibody is designed for detecting RFP fusion protein specifically. Anti-RFP, AlpHcAbs® Human IgG antibody is monovalent, recombinant single domain antibody fused to human IgG1 Fc. Based on immunoelectrophoresis and/or ELISA, Anti-RFP, AlpHcAbs® Human IgG antibody is useful for detecting RFP fusion proteins with high sensitivity.
Immunogen: RFP fusion protein
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to human IgG1 Fc
Conjugate: Unconjugated
Specificity: RFP
Cross-Reactivity: Recognizes mCherry, mRFP, mRFPruby, mPlum, tagRFP, mKate2 and many more RFP derivatives
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles)
Background:
Red fluorescent proteins (RFPs) and variants thereof are widely used to study protein localization and dynamics. RFP can be excited by the 488 nm or 532 nm laser line and is optimally detected at 588 nm.
WB: 1:10,000-1:50,000
ELISA: 1:5000-1:20000
ICC/IF: 1:200-1:1000
IP: 1-2ug/sample
Flow Cyt: 1:200-1:1000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
