SuperVHH™ Anti-Rabbit IgG(H+L), Recombinant Antibody(HRP)

Details and Advantages

                                                Applications:
                                                WB,ELISA
                                            
                                                Reactivity:
                                                Rabbit IgG
                                            
                                                Conjugate:
                                                HRP
                                            
                                                Advantages:
                                                Strict validation
Recombinant Production
Stable Supply
Stable Performance

概述 >

Description:
SuperVHH^TM Anti-Rabbit IgG(H+L), Recombinant Antibody(HRP) is designed for detecting rabbit IgG(H+L) in western blot and ELISA experiment specifically. SuperVHH^TM Anti-Rabbit IgG(H+L), Recombinant Antibody(HRP) is based on recombinant single domain antibodies to mouse IgG(H+L) coupled to HRP. Based on immunoelectrophoresis and/or ELISA, SuperVHH^TM Anti-Rabbit IgG(H+L), Recombinant Antibody(HRP) detects the rabbit IgG, no reactivity with mouse, human, cynomolgus, goat and rat IgG.

SuperVHH^TM Anti-Rabbit IgG(H+L), Recombinant Antibody(HRP) is a mixture of multiple recombinant monoclonal antibodies, which endows this secondary antibody with more controllable properties and more stable performance.

Immunogen: Recombinant rabbit IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: HRP
Specificity: Rabbit IgG(H+L)
Cross-Reactivity: No cross-reactivity with mouse, human, cynomolgus, goat and rat IgG
Purity: Recombinant Expression and Affinity purified
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), Protect from light.

Background:
Rabbit research antibodies are widely used in life science research. So far, four isotypes have been identified (IgA, IgE, IgG, and IgM) in rabbits. Each isotype has a different heavy chain. Rabbit has only one IgG subclass. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains. The heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

性能 >

WB: 1:10000-1:50000
ELISA: 1:10000-1:50000

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.